Research Achievements

Original articles

1. A Competitive AlphaScreen Assay for Detection of Hyaluronan.Huang X, Schmidt TA, Shortt C, Arora S, Asari A, Kirsch T, Cowman MK. Glycobiology. 2017 Dec 29. doi: 10.1093/glycob/cwx109
2. Suppression of Ischemia-Induced Hippocampal Pyramidal Neuron Death by Hyaluronan Tetrasaccharide through Inhibition of Toll-Like Receptor 2 Signaling Pathway.Sunabori T, Koike M, Asari A,Oonuki Y, Uchiyama Y. Am J Pathol.2016 Aug;186(8):2143-51.
3. No influence of exogenous hyaluronan on the behavior of human cancer cells or endothelial cell capillary formation. Seino S, Takeshita F, Asari A,Masuda Y, Kunou M, Ochiya T. J Food Sci. 2014 Jul;79(7):T1469-75.
4. Hyaluronan oligosaccharides perturb lymphocyte slow rolling on brain vascular endothelial cells: implications for inflammatory demyelinating disease.Winkler CW, Foster SC, Itakura A, Matsumoto SG, Asari A, McCarty OJ, Sherman LS.Matrix Biol. 2013 Apr 24;32(3-4):160-8.
5. Oral administration of high molecular weight hyaluronan (900 kDa) controls immune system via Toll-like receptor 4 in the intestinal epithelium.Asari A, Kanemitsu T, Kurihara H. J Biol Chem. 2010 6;285(32):24751-8.
6. Long-term maintenance of liver-specific functions in cultured ES cell-derived hepatocytes with hyaluronan sponge. Teratani T, Quinn G, Yamamoto Y, Sato T, Yamanokuchi H, Asari A, Ochiya T. Cell Transplant.2005;14(9):629-35.
7. Application of fluorophore-assisted carbohydrate electrophoresis to analysis of disaccharides and oligosaccharides derived from glycosaminoglycans. Oonuki Y, Yoshida Y, Uchiyama Y, Asari A.Anal Biochem.2005 15;343(2):212-22.
8. The keratan sulfate disaccharide Gal(6S03) beta1,4-GlcNAc(6S03) modulates interleukin 12 production by macrophages in murine Thy-1 type autoimmune disease. Xu H, Kurihara H, Ito T, Kikuchi H, Yoshida K, Yamanokuchi H, Asari A. J Biol Chem.2005 27;280(21):20879-86.
9. Direct hepatic fate specification from mouse embryonic stem cells. Teratani T, Yamamoto H, Aoyagi K, Sasaki H, Asari A,Quinn G, Sasaki H, Terada M, Ochiya T. Hepatology.2005;41(4):836-46.
10. Isolation and partial characterization of fucan sulfates from the body wall of sea cucumber Stichopus japonicus and their ability to inhibit osteoclastogenesis. Kariya Y, Mulloy B, Imai K, Tominaga A, Kaneko T, Asari A,Suzuki K, Masuda H, Kyogashima M, Ishii T.Carbohydr Res.2004;339(7):1339-46.
11. Specificity of the tumor necrosis factor-induced protein 6-mediated heavy chain transfer from inter-alpha-trypsin inhibitor to hyaluronan: implications for the assembly of the cumulus extracellular matrix. Mukhopadhyay D, Asari A,Rugg MS, Day AJ, Fülöp C. J Biol Chem.2004;279(12):11119-28. Epub 2004 Jan 5. PubMed PMID: 14707130.
12. Differentiation of embryonic stem cells into hepatocytes: biological functions and therapeutic application. Yamamoto H, Quinn G, Asari A,Yamanokuchi H, Teratani T, Terada M, Ochiya T. Hepatology.2003 ;37(5):983-93.
13. Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers. Tawada A, Masa T, Oonuki Y, Watanabe A, Matsuzaki Y, Asari A.Glycobiology.2002;12(7):421-6.
14. Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72. Xu H, Ito T, Tawada A, Maeda H, Yamanokuchi H, Isahara K, Yoshida K, Uchiyama Y, Asari A. J Biol Chem.2002 10;277(19):17308-14.
15. IL-12 enhances lymphoaccumulation by suppressing cell death of T cells in MRL-lpr/lpr mice. Xu H, Kurihara H, Ito T, Nakajima S, Hagiwara E, Yamanokuchi H, Asari A.J Autoimmun.2001;16(2):87-95. Erratum in: J Autoimmun2001;16(4):485.
16. Molecular weight-dependent effects of hyaluronate on the arthritic synovium. Asari A,Miyauchi S, Matsuzaka S, Ito T, Kominami E, Uchiyama Y.Arch Histol Cytol.1998 ;61(2):125-35.
17. Suppression of hyaluronan and prostaglandin E2production in traumatic arthritic synovial cells by sodium hyalruonate.AsariA, Mizuno S, tanaka I, Sunose A, Kuriyama S, Miyazaki K, Namiki O. ConnectiveTissue1997;29:1-5.13.
18. Hyaluronate on heat shock protein and synovial cells in a canine model of osteoarthritis.Asari A,Miyauchi S, Matsuzaka S, Itoh T, Uchiyama Y. Osteoarthritis Cartilage.1996;4(3):213-5.
19. CD44-hyaluronate interaction participates in the adherence of T-lymphocytes to gingival fibroblasts. Murakami S, Saho T, Asari A,Hino E, Kasai D, Shimabukuro Y, Okada H. J Dent Res.1996 ;75(8):1545-52.
20. Human osteoarthritic cartilage exhibits the 2B6 epitope without pretreatment with chondroitinase ABC. Asari A,Akizaki S, Itoh T, Kominami E, Uchiyama Y.Osteoarthritis Cartilage.1996;4(2):149-52.
21. Hyaluronan, CD44 and fibronectin in rabbit corneal epithelial wound healing. Asari A,Morita M, Sekiguchi T, Okamura K, Horie K, Miyauchi S.Jpn J Ophthalmol.1996;40(1):18-25.
22. Cytochemical localization of hyaluronic acid in human synovium with special reference to its possible process of degradation. Asari A,Kuriyama S, Kominami E, Uchiyama Y. Arch Histol Cytol.1995;58(1):65-76.
23. Hyaluronan, cartilage destruction and hydrarthrosis in traumatic arthritis. Asari A,Miyauchi S, Sekiguchi T, Machida A, Kuriyama S, Miyazaki K, Namiki O. Osteoarthritis Cartilage.1994;2(2):79-89.
24. Localization of hyaluronic acid in human articular cartilage. Asari A,Miyauchi S, Kuriyama S, Machida A, Kohno K, Uchiyama Y. J Histochem Cytochem.1994;42(4):513-22.
25. Localization of hyaluronic acid, chondroitin sulfate, and CD44 in rabbit cornea. Asari A,Miyauchi S, Takahashi T, Kohno K, Uchiyama Y. Arch Histol Cytol.1992;55(5):503-11.
26. Intra- and extracellular localization of hyaluronic acid and proteoglycan constituents (chondroitin sulfate, keratan sulfate, and protein core) in articular cartilage of rabbit tibia. Asari A,Miyauchi S, Miyazaki K, Hamai A, Horie K, Takahashi T, Sekiguchi T, Machida A, Kohno K, Uchiyama Y. J Histochem Cytochem. 1992;40(11):1693-704.
27. A morphometric study of the variations in subcellular 　structures of rat hepatocytes during 24 hours. Uchiyama Y, Asari A.Cell Tissue Res.1984;236(2):305-15.

Reviews

1. Potential health benefits of orally administered hyaluronan in alleviating knee joint pain. Tomoyuki Kanemitsu and Akira Asari. In Arthritis (CRC Press) 2011. 
2. Medical application of Hyaluronan. Asari A and Miyauchi https://www.glycoforum.gr.jp/article/04A1.html
3. Novel Functions of Hyaluronan Oligosaccharides. Asari A https://www.glycoforum.gr.jp/article/09A1.html
4. Medical Application of Hyaluronan. Asari A. In Chemistry and Biology of HYALURONAN (ELSEVIER)

Award

2003, Certificate of appreciation from the society of orthopaedist in Japan

Society activity

1. The International Society for Hyaluronan Sciences (ISHAS) Hyaluronan 2007, Hyaluronan 2010、Organizing committee member
2. Hyaluronan Oligosaccharide Workshop 2004 Co-Organizer
3. Hyaluronan Oligosaccharide Workshop 2003 Co-Organizer
4. Hyaluronan Oligosaccharide Workshop 2002 Co-Organizer, Speaker

1. Hyaluronan Tetrasaccharides, HA4 Promotes Stemness and Sirtuin Expression in keratinocytes. Akira Asari, Amina Abbadi, Vincent C. Hascall. Hyaluronan 2015, Florence, Italy.
2. Hyaluronan Tetrasaccharides, HA4 Promotes Stemness and Sirtuin Expression. Gordon Research Conference, Proteoglycans, Diverse Regulators of Health and Disease. Akira Asari, Vincent C. Hascall. 6-11, 2014, Proctor Academy, Andover, NH. Invited speaker.
3. Effects of Hyaluronan Tetrasaccharides, HA4 on Stemness and Corneal Wound Healing. Akira Asari, Vincent C. Hascall. Gordon Research Conference, 
Cornea, Biology & Pathobiology of the Cell Lineage and Fate Determination on the Ocular Surface During 
Development and Disease. 16-21, 2014, Ventura Beach Marriott, Ventura, CA. Invited speaker.
4. Effect of oral administration of high-molecular weight hyaluronan on carrageenan-induced changes related with inflammatory pain in rats. Hyaluronan 2013, Oklahoma, USA. Invited speaker.
5. Oral administration of high molecular weight hyaluronan (900 kDa) controls immune system via toll-like receptor 4 in the intestinal epithelium. World Vaccine Congress, 2011. Invited speaker.
6. Oral administration of high molecular weight hyaluronan (900 kDa) controls immune system via toll-like receptor 4 in the intestinal epithelium. Gordon Research Conference Proteoglycans, 2010. Invited speaker.
7. Oral administration of high molecular weight hyaluronan (900 kDa) controls immune system via toll-like receptor 4 in the intestinal epithelium.Hyaluronan 2010 the International Society for Hyaluronan Sciences. Invited Speaker, Co-Chair, Program Committee member
8. Multi-functions of hyaluronan tetrasaccharides. Hyaluronan 2007 the International Society for Hyaluronan Sciences. Invited Speaker, Co-Chair, Program Committee member
9. Effects of hyaluronan tetrasaccharides on spinal cord injury. Gordon Research Conference proteoglycans 2004. Invited Speaker
10. Effects of keratan sulfate disaccharides on MRL-lpr/lprmice. Gordon Research Conference proteoglycans 2004. Invited Speaker
11. Effects of tetrasaccharides of hyaluronan on heat shock protein. Hyaluronan 2000 the International Society for Hyaluronan Sciences. Speaker
12. Effects of tetrasaccharides of hyaluronan on heat shock protein and cell death. International Conference on Hyaluronan 1999